• 概述

• 产品描述 Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins.
• 产品名称 Anti-Myc Tag Mouse Monoclonal Antibody [A3-B4]
• 验证应用 WB,IF-Cell,ELISA,FC,IP
• 抗体类型 小鼠单抗
• 免疫原 Synthetic peptide immune sequence is EQKLISEEDLC-KLH.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 2 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG2b
• 纯化方式 Protein G affinity purified.
• 其它名称
  • avian myelocytomatosis viral oncogene homolog antibody
  • bHLHe39 antibody
  • c-Myc antibody
  • class E basic helix-loop-helix protein 39 antibody
  • MRTL antibody
  • MYC antibody
  • Myc Epitope Tag antibody
  • myc proto-oncogene protein antibody
  • myc-related translation/localization regulatory factor antibody
  • oncogene c-Myc antibody
  • proto-oncogene c-Myc antibody
  • protooncogene homologous to myelocytomatosis virus antibody
  • transcription factor p64 antibody
  • v-myc avian myelocytomatosis viral oncogene homolog antibody
  • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
  • MycTag
  more

• 应用

  WB: 1:2,000
  IF-Cell: 1:50
  FC: 1:50-1:100
  IP: 2-5 µg/ml.

• 

  Fig1: Western blot analysis of Myc Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: C-terminal Myc-tagged recombinant protein
  Lane 2: N-terminal Myc-tagged recombinant protein

  

  Fig2: ICC staining of Myc Tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM31105, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.
  
  Lane 1: Myc Tag fusion protein lysate (input).
  Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
  Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
  Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.
  
  Blocking/Dilution buffer: 5% NFDM/TBST

  

  Fig4: Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.
  
  Lane 1: Myc Tag fusion protein lysate (input).
  Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
  Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
  Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.
  
  Blocking/Dilution buffer: 5% NFDM/TBST

• 背景文献

• 1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
  
  2. "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci. U.S.A. 90:3216-3220(1993)

• Publishing research using Anti-Myc Tag Mouse Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  35800772 LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination Author: Jiang, J., Li, Y., Sun, Z., Gong, L., Li, X., Shi, F., Yao, J., Meng, Y., Meng, X., Zhang, Q., Wang, Y., Su, X., & Diao, H. Published: 2022 Application: WB Journal: iScience IF: 5.458
• PMID:
  35806389 CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation Author: Chen, H., Hu, K., Xie, Y., Qi, Y., Li, W., He, Y., Fan, S., Liu, W., & Li, C. Published: 2022 Application: WB Journal: Int J Mol Sci. IF: 5.924
• PMID:
  36314827 IRF2 Cooperates with Phosphoprotein of Spring Viremia of Carp Virus to Suppress Antiviral Response in Zebrafish Author: Huang, W., Zhao, X., Ji, N., Guo, J., Feng, J., Chen, K., Wu, Y., Wang, J., Feng, H., & Zou, J. Published: 2022 Application: WB Species: Zebrafish Journal: Oncologist. IF: 5.837
• PMID:
  33469231 Site-1 protease controls osteoclastogenesis by mediating LC3 transcription. Cell death and differentiation, 28(6), 2001–2018. Author: Zheng, Z., Zhang, X., Huang, B., Liu, J., Wei, X., Shan, Z., Wu, H., Feng, Z., Chen, Y., Fan, S., Zhao, F., & Chen, J. Published: 2021 Application: WB,IF,IP Species: Mouse Journal: Cell Death Differ IF: 10.717
• PMID:
  30366764 Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages Author: 王迪 Published: 2018 Application: WB Species: None Journal: Immunity. IF: 19.734

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.