Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA tag recombinant protein.
Anti-HA tag Recombinant Rabbit Monoclonal Antibody
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
- hemagglutinin antibody
- Hemagglutinin HA1 chain antibody
- Hemagglutinin HA2 chain antibody
IP: 2-5 µg/ml.
Fig1: Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: C-terminal HA-tagged recombinant protein
Lane 2: N-terminal HA-tagged recombinant protein
Fig3: HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with ET1611-49 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.
Lane 1: HA Tag fusion protein lysate (input).
Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).
2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).
using Anti-HA tag Recombinant Rabbit Monoclonal Antibody? Please let us know so
that we can cite the reference in this datasheet.
The Zscan4-Tet2 Transcription Nexus RegulatesMetabolic Rewiring and Enhances Proteostasis toPromote Reprogramming
Author: Mingliang Zhang;Dan Ye Published: 2020 Application: IP,WB Species: human Journal: Cell Reports IF: 8.109
Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1.
Author: Linrong Lu Published: 2017 Application: WB Species: mouse Journal: J Exp Med IF: 11.991
Choose your product,application and species below,then click
On the next page you can complete and submit your review.
Leave us a review
To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you
Click here to view the