• 概述

• 产品描述 Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA tag recombinant protein.
• 产品名称 Anti-HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]
• 验证应用 WB,IP
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 其它名称
  • HA epitope tag antibody
  • HA1 antibody
  • HA2 antibody
  • hemagglutinin antibody
  • Hemagglutinin HA1 chain antibody
  • Hemagglutinin HA2 chain antibody
  • HAtag
  more

• 应用

  WB: 1:1,000-1:5,000
  IP: 2-5 µg/ml.

• 

  Fig1: Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: C-terminal HA-tagged recombinant protein
  Lane 2: N-terminal HA-tagged recombinant protein

  

  Fig3: HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with ET1611-49 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.
  
  Lane 1: HA Tag fusion protein lysate (input).
  Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
  Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
  Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate.
  
  Blocking/Dilution buffer: 5% NFDM/TBST

• 背景文献

• 1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).
  
  2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).

• Publishing research using Anti-HA tag Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  32668244 The Zscan4-Tet2 Transcription Nexus RegulatesMetabolic Rewiring and Enhances Proteostasis toPromote Reprogramming Author: Mingliang Zhang;Dan Ye Published: 2020 Application: IP,WB Species: human Journal: Cell Reports IF: 8.109
• PMID:
  28400474 Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. Author: Linrong Lu Published: 2017 Application: WB Species: mouse Journal: J Exp Med IF: 11.991

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.