• Overview

• Introduction Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay. This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest. Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein.GST Nanoselector Agarose is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.The GST-Trap consists of a GST Nanobody /VHH coupled to agarose beads.
• Target/Specificity Glutathione S-Transferase (GST) from Schistosoma japonicum
• Affinity 0.26nM
• Bead properties Bead size: ~ 90 µm Storage buffer: 20% EtOH
• Binding capacity High binding capacity,10 µL slurry bind about 20 µg of recombinant GST
• Elution - SDS sample buffer - 0.2 M glycine pH 2.5 Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.
• Stability and Storage Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.
• Benefits Effective pull-down of GST fusion proteins, low background Significantly higher sensitivity than glutathione cellulose resin and other affinity resins NO heavy & light chain contamination High binding affinity Harsh washing conditions

• 

  Fig 1: Immunoprecipitation of GST protein With GST Nanoselector Agarose.
  
  Lane 1：Input
  Lane 2: Flow-through
  Lane 3: PH=2 Elution
  Lane 4: Eluted Beads

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.