• 概述

• 产品描述 Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.
• 产品名称 Anti-Beta Actin Rabbit Polyclonal Antibody
• 分子量 Predicted band size: 42 kDa
• 种属反应性 Human,Mouse,Rat,Zebrafish,bamboo
• 验证应用 WB,IF-Cell,IHC-P,FC
• 抗体类型 兔多抗
• 免疫原 Synthetic peptide within N-terminal residues of β-Actin.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Immunogen affinity purified.
• 亚细胞定位 Cytoskeleton
• 数据链接 SwissProt: P60709 Human
  SwissProt: P60710 Mouse
  SwissProt: P60711 Rat
  SwissProt: Q7ZVI7 Zebrafish
  
• 其它名称
  • A26C1A antibody
  • A26C1B antibody
  • ACTB antibody
  • ACTB_HUMAN antibody
  • Actin beta antibody
  • Actin cytoplasmic 1 antibody
  • Actin, cytoplasmic 1, N-terminally processed antibody
  • Actx antibody
  • b actin antibody
  • Beta cytoskeletal actin antibody
  • Beta-actin antibody
  • BRWS1 antibody
  • E430023M04Rik antibody
  • MGC128179 antibody
  • PS1TP5 binding protein 1 antibody
  • PS1TP5BP1 antibody
  • β-actin antibody
  • β actin antibody
  • BetaActin
  more

• 应用

  WB: 1:10,000

• 

  Fig1: Western blot analysis of Beta Actin on different lysates with Rabbit anti-Beta Actin antibody (R1207-1) at 1/10,000 dilution.
  
  Lane 1: HeLa cell lysate
  Lane 2: A549 cell lysate
  Lane 3: Jurkat cell lysate
  Lane 4: NIH/3T3 cell lysate
  Lane 5: PC-12 cell lysate
  Lane 6: Mouse brain tissue lysate
  Lane 7: Rat brain tissue lysate
  Lane 8: Mouse lung tissue lysate
  Lane 9: Rat lung tissue lysate
  
  Lysates/proteins at 20 µg/Lane.
  
  Predicted band size: 42 kDa
  Observed band size: 42 kDa
  
  Exposure time: 30 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1207-1) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of β-actin on different lysates using anti-β-actin antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: hybrid fish (crucian-carp) brain tissue
  Lane 2: hybrid fish (crucian-carp) kidney tissue

• 背景文献

• 1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L."Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA." Nucleic Acids Res. 12:1687-1696(1984) Ohmori H., Toyama S., Toyama S."Direct proof that the primary site of action of cytochalasin on cell motility processes is actin."J. Cell Biol. 116:933-941(1992)

• Publishing research using Anti-Beta Actin Rabbit Polyclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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• PMID:
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• PMID:
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• PMID:
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• PMCID:
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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.